JEE Main · 2019 · Shift-IImediumBIO-118

Which of the following tests cannot be used for identifying amino acids?

Biomolecules · Class 12 · JEE Main Previous Year Question

Question

Which of the following tests cannot be used for identifying amino acids?

Options
  1. a

    Biuret test

  2. b

    Barfoed test

  3. c

    Ninhydrin test

  4. d

    Xanthoproteic test

Correct Answerb

Barfoed test

Detailed Solution

Step 1: Reviewing Common Tests — Ninhydrin and Biuret are standard tests for amino acids/proteins. Xanthoproteic identifies aromatic amino acids.

Step 2: Carbohydrate TestingBarfoed’s test\text{Barfoed's test} is specifically designed to distinguish between monosaccharides and disaccharides.

Step 3: Selection — Since Barfoed’s test\text{Barfoed's test} is exclusive to sugars, it cannot be used to identify amino acids. Correct option is (b).

Key Point: The Biuret test\text{Biuret test} requires at least two peptide bonds (tripeptides and larger).

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Which of the following tests cannot be used for identifying amino acids? (JEE Main 2019) | Canvas Classes