Which one among the following chemical tests is used to distinguish monosaccharide from disaccharide?
Biomolecules · Class 12 · JEE Main Previous Year Question
Which one among the following chemical tests is used to distinguish monosaccharide from disaccharide?
- a
Seliwanoff's test
- b
Iodine test
- c✓
Barfoed test
- d
Tollen's test
Barfoed test
Step 1: Specificity of Barfoed's Test — uses copper acetate in acetic acid. Both monosaccharides and reducing disaccharides react, but at different rates.
Step 2: Identifying Difference — Monosaccharides react much faster (precipitate within minutes), while disaccharides take longer or require more vigorous heating.
Step 3: Final Choice — Because of the significant difference in reaction time, this test is used to distinguish the two classes. Correct option is (c).
Remember: Each carbohydrate test has a unique set of conditions that targets certain structural features.
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