A, B and C are three biomolecules. The results of the tests performed on them are given below:

| | Molisch's test | Barfoed Test | Biuret Test |
|---|---|---|---|
| A | Positive | Negative | Negative

Question

A, B and C are three biomolecules. The results of the tests performed on them are given below:

| | Molisch's test | Barfoed Test | Biuret Test |
|---|---|---|---|
| A | Positive | Negative | Negative |
| B | Positive | Positive | Negative |
| C | Negative | Negative | Positive |

A, B and C are respectively:

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Test analysis:

Molisch's test: Positive for ALL carbohydrates (both mono and polysaccharides).
Barfoed's test: Positive for monosaccharides only (not disaccharides). Distinguishes mono from disaccharides.
Biuret test: Positive for proteins (peptide bonds, –CO–NH–).

Interpreting results:

| Compound | Molisch | Barfoed | Biuret | Conclusion |
|---|---|---|---|---|
| A | + | − | − | Carbohydrate, but NOT monosaccharide → Disaccharide |
| B | + | + | − | Carbohydrate AND monosaccharide → Monosacc

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