If one strand of a DNA has the sequence ATGCTTCA, sequence of the bases in complementary strand is:

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DNA base pairing rules (Chargaff's rules):

| Base in template strand | Complementary base |
|---|---|
| A (Adenine) | T (Thymine) |
| T (Thymine) | A (Adenine) |
| G (Guanine) | C (Cytosine) |
| C (Cytosine) | G (Guanine) |

Complementary strand is antiparallel — read 3'→5' when the template is 5'→3'.

Template strand: 5'–A–T–G–C–T–T–C–A–3'

Complementary strand: 3'–T–A–C–G–A–A–G–T–5'

Reading the complementary strand 5'→3': T–A–C–G–A–A–G–T

Final Answer: Option (2) — TACGAAGT

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