The structure of protein that is unaffected by heating is :

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Effect of heating on protein structure:

Heating disrupts non-covalent interactions that maintain higher-order protein structures:

| Structure | Stabilized by | Effect of heating |
|---|---|---|
| Primary | Covalent peptide bonds (–CO–NH–) | Unaffected ✓ |
| Secondary | H-bonds (-helix, -sheet) | Disrupted |
| Tertiary | H-bonds, disulfide bonds, hydrophobic, ionic | Disrupted |
| Quaternary | Non-covalent interactions between subunits | Disrupted |

The primary structure consists of amino acid

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